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Team:Bielefeld-CeBiTec/Results/DataPage
From 2014.igem.org
Data Page
This page gives a basic overview about our project The Transformers - From Carbon Dioxide To Biofuel. A detailed description of our subprojects can be found in our project part of the Wiki.
Figure 1: Project description
Data for rMFC
| BioBrick | Description |
|---|---|
| BBa_K1465101 | Cytochrome c from Geobacter sulfurreducens for electron transfer with mediators for reduction equivalent generation |
| BBa_K1465102 | Fumerate reductase (frd) from E. coli under the control of the T7 promoter |
| BBa_K1465103 | Fumerate reductase (FumABCD) from Actinobacillus succinogenes |
| BBa_K1465104 | Fumarate reductase FumBCD from Actinobacillus succinogenes |
| BBa_K1465105 | Fumarate reductase (Fum) under control of the T7 promoter |
| BBa_K1465107 | Primer sequence for genome editing of porine fwd |
| BBa_K1465108 | Primer sequence for genome editing of porine rev |
| BBa_K1465109 | Primer sequence for genome editing of dcuB fwd |
| BBa_K1465110 | Primer sequence for genome editing of dcuB rev |
Data for CO2 Fixation
| BioBrick | Description |
|---|---|
| BBa_K1465201 | Phosphoribulokinase prkA from Synechoccus elongatus, codon optimized for E. coli. |
| BBa_K1465202 | Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Halothiobacillus neapolitanus |
| BBa_K1465203 | Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) from Synechoccus elangatus. |
| BBa_K1465204 | Shell-associated protein (csoS2) of the carboxysome from Halothiobacillus neapolitanus. |
| BBa_K1465205 | Carbonic anhydrase (csoS3) of the carboxysome from Halothiobacillus neapolitanus. |
| BBa_K1465206 | shell proteins (csoS4) of the carboxysome from Halothiobacillus neapolitanus. |
| BBa_K1465207 | pore-forming protein (csoS1D) of the carboxysome from Halothiobacillus neapolitanus. |
| BBa_K1465208 | can + shell proteins (cloning intermediate) from Halothiobacillus neapolitanus |
| BBa_K1465209 | Carbonic anhydrase + shell proteins + csoS1D from Halothiobacillus neapolitanus |
| BBa_K1465212 | Phosphoribulokinase prkA from Synechoccus elongatus under the control of the ptac promotor |
| BBa_K1465213 | RubisCO from Halothiobacillus neapolitanus under control of the ptac promoter |
| BBa_K1465214 | Phosphoribulokinase of Halothiobacillus neapolitanus under control of ptac |
| BBa_K1465222 | shell-protein fused with GFP |
| BBa_K1465223 | Empty Carboxysome with GFP |
| BBa_K1465225 | Knock-down with sRNA of phosphofructo kinase. (sRNA:pfkA) |
| BBa_K1465226 | Knock-down with sRNA of phosphofructo kinase under control of ptac promotor (ptac_sRNA:pfkA) |
| BBa_K1465227 | Knock-down with sRNA of phosphofructo kinase under control of T7 promotor. (t7_sRNA:pfkA) |
| BBa_K1465228 | Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus |
| BBa_K1465229 | Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus under the control of the ptac promotor |
| BBa_K1465230 | Purification vector (T7-RBS Intein-tag) |
Data for Isobutanol
| BioBrick | Description |
|---|---|
| BBa_K1465301 | Alochol dehydrogenase from Lactococcus lactis |
| BBa_K1465302 | Isobutanol producing pathway (alsS_ilvC_ilvD_kivD) |
| BBa_K1465303 | Isobutanol producing pathway combined with alcohol dehydrogenase (alsS_ilvC_ilvD_kivD_adhA) |
| BBa_K1465304 | Alcohol dehydrogenase under control of T7 promotor |
| BBa_K1465305 | Alcohol dehydrogenase under control of ptac promotor |
| BBa_K1465306 | Isobutanol producing pathway under control of ptac promotor |
| BBa_K1465307 | Isobutanol producing pathway combined with alcohol dehydrogenase under control of ptac promotor |
Data for Biosafety
| BioBrick | Description |
|---|---|
| BBa_K1465401 | Alanin racemase (alr) knock-out strain fused with RFP as visual control for transformation in E.coli |
| BBa_K1465403 | Primer for alanin racemase (alr) fwd |
| BBa_K1465404 | Primer for alanin racemase (alr) rev |
| BBa_K1465405 | Primer for dadX fwd |
| BBa_K1465406 | Primer for dadX rev |
