Because our Trogene Horse use phages as vector, it is very important to know how to make the best of these phages. This means we need to get a condition under which these phages can work most efficiently. In our experiment, the phage display can be descripted as: Prepare E.coli JM101 with phagemid→ Add M13KO7 to infect JM101 → Add kanamycin for selection → Incubate for phagemid-carrying phage releasing → Use phagemid-carrying phage infect blank JM101. Thus, we chose two events as characters of phage’s efficiency:
1. The release amount of phage after helper phage infection. 2. The infection rate of our phagemid-carrying phage
So we collected some experimental data for modeling. And here comes the result. We wolud like to give our appreciation to NCTU-Formosa, they taught us how to make this modeling.
MATLAB
MATLAB(MATrix LABoratory) is a high-level language and interactive environment for numerical computation, visualization, and programming. Using MATLAB, you can analyze data, develop algorithms, and create models and applications. The language, tools, and built-in math functions enable you to explore multiple approaches and reach a solution faster than with spreadsheets or traditional programming languages, such as C/C++ or Java™.
ANFIS The architecture and learning procedure underlying ANFIS (Adaptive Network-based Fuzzy Inference System) is presented, which is a fuzzy inference system implemented in the framework of adaptive networks. By using a hybrid learning procedure, the proposed ANFIS can construct an input-output mapping based on both human knowledge (in the form of fuzzy if-then rules) and stipulated input-output data pairs. In the simulation, the ANFIS architecture is employed to model nonlinear functions, identify nonlinear components on-line in a control system, and predict a chaotic time series, all yielding remarkable results. Comparisons with artificial neural networks and earlier work on fuzzy modeling are listed and discussed.
To build the model,we created, trained, and tested Fuzzy Inference System(FIS).We performed the following tasks:
1.Data pre-processing 2.Loading the Data 3.Generating the Initial FIS Structure 3.Training the FIS 4.Validating the Trained FIS
Data pre-processing
Loading the Data
To train the FIS, we must begin by loading a Training data set that contains the desired input/output data of the system to be modeled. Any data set we load must be an array with the data arranged as column vectors, and the output data in the last column.We also loaded Testing data.
Generating the Initial FIS Structure
Before we start the FIS training, we specify an initial FIS model structure as below:
Training the FIS
T
Validating the Trained FIS
Model 1 : Release
We chose two variables to find the best release amount of phage after helper phage infecting. The first variable is the time after we input helper phages M13KO7 into E.Coli (JM101) with phagemid pBluescript II SK(-).(We have put BBa_I13521 inside as an reporter gene.)This is the time for helper phages to infect bacterium. After the infection, we added kanamycin into these JM101 for selection becauseJM101 can get kanamycin resistance only when they are infected by M13KO7. Then we incubated these JM101 so they can have time to release phagemid-carrying phage, and the incubating time is the second variable in our test. As we can see in this figure, the most amount of phage being released is at the time when we add kanamycin after 30 minutes of infection and then incubate them for 14 hours. Under this condition, the best releasing amount of phage is 4×1010 pfu/ml.
This modleing is been helper by NCTU-Formosa. They analyzed our data and made this modeling.
Thanks NCTU-Formosa!!! You are our best friend!
Then we came up with another question: Under which condition will we get the best infection rate of our phagemid-carrying phage? We believe this is influenced by the MOI between phages and bacterium. MOI means multiplicity of infection, it is the ratio of agents (phage-carrying phage) to infection targets (E.coli JM101). So we choose MOI as the variable, and incubate bacterium for 1 hour after infecting. In our test, we found that as long as the MOI is higher than 6 pfu/cfu, the infection rate can access 100%. This is an exciting result!!
Lost the way? Use it to help you if you're lost.
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