Bio Bricks

BBa_K1470000: mCat-1

mCat-1 is the receptor we introduced to human cells to be able to infect them using the MuLV

BBa_K1470001: Puromycin N-acetyl-transferase

Puromycin N-acetyl-transferase is an enzyme which we used for selecting stable eucaryotic cell lines. Because of the blocked protein biosynthesis more than 99% of uninfected cells will be dead within two days.

BBa_K1470002: Galactose-induced gene 4 (Gal4BD)

Galactose-induced gene 4 (Gal4BD) is used to investigate gene expression levels.

BBa_K1470003: Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE)

Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element (WPRE) is a regulatory RNA sequence, which promotes viral gene expression.

BBa_K1470004: Secreted Alkaline Phosphatase (SEAP)

Secreted Alkaline Phosphatase (SEAP) is used in cell culture assays to quantify gene expression.

BBa_K1470005: engineered PDZ domain (ePDZ)

engineered PDZ domain (ePDZ) is an important part of the blue light system.

BBa_K1470006: The Viral Vector (pMIG)

The Viral Vector (pMIG) is the plasmid which is used to tranfect Phoenix cells with the gene to be packed into viral particles. It contains two LTRs and a multiple cloning site.