Team:INSA-Lyon/Parts

5 biobricks have been constructed, three for Nickel chelation, three for promoter characterization.

The principal of the construction is shown in the figure below.

On a pSB1C3 backbone, two essential part have been assembled: a promoter and a reporter coding for CsgA.The promoter in this case is always the same: P70.However, the reporter is different for each construction.
The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA.
The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called His1.
The last CsgA is almost the same but with 2 his tag. This construction is called His2.
5 biobricks have been constructed, three for Nickel chelation, three for promoter characterization.
Biobrick for promoter characterization
The principal of the construction is shown in the figure below.

On a pKK backbone, two essential parts have been assembled: a promoter and a reporter.
The reporter in this case is always the same: gfp. However, the promoter is different for each construction.
The first promoter is P70, sequence found in the Resgistry. This construction is called p70::gfp
The second promoter is PCurli, obtained from a PCR, sequence coding for the inter-genic regulation for curli production. This construction is called pcurli::gfp.