Team:INSA-Lyon/Parts

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Curly'on - IGEM 2014 INSA-LYON

  • 5 biobricks have been constructed, three for Nickel chelation, three for promoter characterization.




    Biobricks for nickel chelation

    The principal of the construction is shown in the figure below.





  • On a pSB1C3 backbone, two essential part have been assembled: a promoter and a rapporter coding for CsgA.The promoter in this case is always the same: P70.However, the rapporter is different for each construction.

  • The first CsgA is called CsgA WT, the sequence is exactly the same as we found in a wild type CsgA protein except one mutation in order to eliminate one restriction site. The construction is also called CsgA.




  • The second CsgA is based on the same sequence but with a short sequence coding for a his tag(a peptide chain coding six histidines). This construction is called His1.




  • The last CsgA is almost the same but with 2 his tag. This construction is called His2.




    • <groupparts>iGEM014 INSA-Lyon</groupparts>

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