Luria-Bertani culture medium + Mercury chloride (ML) production - Low Salt

Reagents:

1. 5 grams of NaCl.

2. 10 grams of Peptone.

3. 5 grams of yeast extract.

Reagents for solid medium:

1. 5 grams of NaCl.

2. 10 grams of Peptone.

3. 5 grams of yeast extract.

4. 15 grams of Bacteriological Agar.

Steps:

1. Dissolve all the reagents in distilled water to 1 liter.

2. Autoclave for 15 minutes at 120 °C.

3. Add required amount of mercury chloride to the experiment.