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Team:NJU-QIBEBT/humanPractice
From 2014.igem.org
Human Practice
1. Meetup at Huazhong Agricultural University
On 23rd and 24th of August, we participated in a sharing conference established by Huazhong Agricultural University. Zhou Yu, Qin Zhen, Rong Hui and Zhang Qiuhao from our team went to Wuhan and met up with students from Peking University, Sun Yat-Sen University, Wuhan University and some other universities. We gave a presentation about our project and also got to know what other teams were busy with. An internet-based group was set up for further discussion. Another team from Wuhan focuses on the fatty acid as well, thus our two teams shared experience for a mutual progress. Although some of the team’s project is quite different from ours, their experience about problems solving is still valuable to us.
2. Internship in Qingdao Institute of Bioenergy and Bioprocess Technology, CAS
In late August, 3 members of our team, Zhou Yu, Rao Bing and Zhu Ruichi, went to Qingdao Institute of Bioenergy and Bioprocess Technology for the function test of the plasmids we built. They conducted a few experiments there including extracting fatty acids and examine the species of fatty acids under the guidance of the PhD students there. One of the significant experiment they did there was they applied fermenter to cultivate a huge amount of E.coli for more quantity of fatty acids.
3. Small-scale Industrialization
After the experiments in Qingdao Institute of Bioenergy and Bioprocess Technology, Zhou Yu went to the factory in Qingdao with our project. He tried to apply what we studied to practice. The technician taught him some experience on fermentation. At the same time, he also learned something from the industry and managed to apply to our project. Huge fermenters were used for fermentation in a large scale.
4. Establishment of iGEM Club in Nanjing University
A club named iGEM was formed by our team and another team ’Nanjing-China’ in our campus. We can publicize the idea of the iGEM competition to other students and thus it can attract more students to join this competition. Furthermore, this club intends to recruit some members from other departments and our team can therefore be more versatile. Anyway, the establishment of this club will enable the iGEM competition to continue at our university in the future.
Attribution
Project
Production increase and control module
Isolation of promoter and operator sequences from lac operon and ara operon: Xu Chunhui, Wen Yu Qi, Zhang Qiuhao and Zhao Yiling
Isolation of promoter and operator sequences from trp operon: Xu Yilin, Chen Yuda, Yang Zhanzhao
Plasmid construction with lac operon, ara operon, AtfatA and bte: Qin Zhen, Rao Bing, Rong Hui, Zhou Yu and Zhu Yi
Plasmid construction with trp operon, fadA and fadB: Xu Yilin, Chen Yuda, Yang Zhanzhao and Zhu Qizhang
QPCR: Zhu Yi, Qin Zhen, Zhou Yu, Xu Yilin
Plasmid function test in QIEBET: Rao Bing, Zhu Ruichi and Zhou Yu
Advisor: Liu Yanqing, Wang Xueliang
Pumping out module
Plasmid construction with msbA and pfadD: Qin Zhen, Rao Bing, Rong Hui, Zhu Yi and Zhu Ruichi
QPCR: Rao Bing, Rong Hui
Plasmid function test in QIEBET: Rao Bing, Zhu Ruichi and Zhou Yu
Advisor: Liu Yanqing, Wang Xueliang
Indicator module
Plasmid construction: Ou Yangli
Advisor: Xiong Aoli
Plasmid function test: Rao Bing, Zhu Ruichi and Zhou Yu
QPCR: Ou Yangli, Zhang Qiuhao, Xu Chunhui
Modeling
Data analysis: Ou Yangli, Zhao Yiling,
Construction: Bu Jinzhi
Painting
Xu Yilin, Qin zhen
Wiki
Designer:
Parts, protocol and notebook collection: Xu Chunhui, Zhang Qiuhao, Yang Zhanzhao, Chen Yuda, Zhu Qizhang, Zhao Yiling, Wen Yuqi and Ou Yangli
Editor: Zhu Yi, Zhu Ruichi, Rong Hui, Zhang Qiuhao
Subeditor: other team members
Human Practice
Meetup in Wu Han: Qin Zhen, Rong Hui, Zhang Qiuhao and Zhou Yu
Internship in QIBEBT: Rao Bing, Zhu Ruichi and Zhou Yu
Small-scale industrialization: Zhou Yu
iGEM Club establishment in Nanjing University: Yang Zhanzhao, Wen Yuqi
