Team:StanfordBrownSpelman/Lab

Lab Techniques, Special
Protocols, & Project Journals

General Protocols

Here are links to pages documenting all of our laboratory protocols and procedures, from general lab techniques to special protocols we developed while working on our biological UAV project. For general laboratory protocols with an emphasis on NASA Ames Research Center lab practice, check out the iGEMer's Guide to the Galaxy.

● Using the autoclave, media, and generating antibiotic sticks

● Plates, Liquid Cultures, and Cryostocking

● Miniprep, Nanodrop, and Digestion

● Gel Casting, Loading, Extraction, and Cleanup

● Ligation and Chemically Competent Transformation

● Everything Protein Gel Related

● Everything PCR (Including Gel Extraction)

● Primers and Sequencing

● Using the iGEM Parts Registry

● Culturing Bacillus subtilis

● Site Directed Mutagenesis

New Protocols for 2014

Our projects this year required a few specific protocols that aren't covered in our iGEMer's Guide to the Galaxy. Check them out here!