Team:BUCT-China
From 2014.igem.org
PROJECT
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OVERVIEW
There are a population of 884 million still drink water without the previous purification all over the world...
BIOSENSORS
Quorum sensing was first found in Vibrio fischeri in the ocean. Microorganisms achieve information communication by ...
DEVICE
Adoption of luminescent bacteria for air pollution monitoring, antibiotics screening and rapid...
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Adoption of luminescent bacteria for air pollution monitoring, antibiotics screening and rapid...
Project: The influence of temperature on the engineering bacteria in the fermentation tank
23rd Mar 2014 Meet Up with BIT-iGEM
Encountered Problems:
1、 too complicated gene pathway to accomplish in a short period of time;
2、 the effects of Web site, poster and other artists are not so beautiful;
3、 time for Sponsorship and advertising is a bit tight;
4、 visa and travel arrangements should be considered early.
Notice:
1、 DNA distribution must be tested before you intend to use one of those parts;
2、 Meet up between teams and parts interchange needed to be paid attention to;
3、 Souvenirs are recommended;
4、 Preparation of team T-shirt and mascot;
5、 Pamphlets are really necessary , portable, convenient and practical .
Help Issues:
1、 the bacterial genes and genetic circuits implementation;
2、 some help about experimental skills and techniques;
3、 can provide temperature sensitive strains if needed.
Project: The influence of temperature on the engineering bacteria in the fermentation tank
23rd Mar 2014 Meet Up with BIT-iGEM
Encountered Problems:
1、 too complicated gene pathway to accomplish in a short period of time;
2、 the effects of Web site, poster and other artists are not so beautiful;
3、 time for Sponsorship and advertising is a bit tight;
4、 visa and travel arrangements should be considered early.
Notice:
1、 DNA distribution must be tested before you intend to use one of those parts;
2、 Meet up between teams and parts interchange needed to be paid attention to;
3、 Souvenirs are recommended;
4、 Preparation of team T-shirt and mascot;
5、 Pamphlets are really necessary , portable, convenient and practical .
Help Issues:
1、 the bacterial genes and genetic circuits implementation;
2、 some help about experimental skills and techniques;
3、 can provide temperature sensitive strains if needed.
Project: The influence of temperature on the engineering bacteria in the fermentation tank
23rd Mar 2014 Meet Up with BIT-iGEM
Encountered Problems:
1、 too complicated gene pathway to accomplish in a short period of time;
2、 the effects of Web site, poster and other artists are not so beautiful;
3、 time for Sponsorship and advertising is a bit tight;
4、 visa and travel arrangements should be considered early.
Notice:
1、 DNA distribution must be tested before you intend to use one of those parts;
2、 Meet up between teams and parts interchange needed to be paid attention to;
3、 Souvenirs are recommended;
4、 Preparation of team T-shirt and mascot;
5、 Pamphlets are really necessary , portable, convenient and practical .
Help Issues:
1、 the bacterial genes and genetic circuits implementation;
2、 some help about experimental skills and techniques;
3、 can provide temperature sensitive strains if needed.
quorum sensing system
Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can ...
Fast-type merR biosensor
merR gene acts as a regulator which can promote downstream genes’ expression in the presence of Hg (II), the detailed mechanism of it is well-known as a whole...
Hg(II)
sensor
We built our part with an existed part----which provided us a PmerT promoter----and a merR sequence synthetized by ourselves.However, you need to add upstream an rbs to make it work if you decide to adopt our part....
K1513000: quorum sensing system
Our part is constituted by Plac、luxR、Pluxr、luxr. LuxI can result in the production of AHL—a kind of auto-inducer which can bind to the protein LuxR and induce the high expression of downstream genes of Pluxr,therefore,this system can drive a mass of bacteria highly express a particular gene. What’s more, AHL can be used by all bacteria in certain range.
K1513001: Fast-type merR biosensor
merR gene acts as a regulator which can promote downstream genes’ expression in the presence of Hg (II), the detailed mechanism of it is well-known as a whole. This summer we aimed at finding out a fast as well as accurate method to detect the Hg (II) in water. However, we found that the former method costs much time, so we turn to look for a rapid way which meets our expectation.
K1513002:Hg(II) sensor
We built our part with an existed part----which provided us a PmerT promoter----and a merR sequence synthetized by ourselves. However, you need to add upstream an rbs to make it work if you decide to adopt our part.
HP
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Meet Up with BIT-iGEM
23rd Mar. 2014
The influence of temperature on the engineering bacteria in the fermentation tank
Meet Up with BIT-China
3rd May 2014
Microfluidic technology application of antibiotics detection in the milk
Briefing Session in university
21st Sep. 2014
Introduction of the concept of Synthetic Biology and the meaning of iGEM
Project: The influence of temperature on the engineering bacteria in the fermentation tank
23rd Mar 2014 Meet Up with BIT-iGEM
Encountered Problems:
1、 too complicated gene pathway to accomplish in a short period of time;
2、 the effects of Web site, poster and other artists are not so beautiful;
3、 time for Sponsorship and advertising is a bit tight;
4、 visa and travel arrangements should be considered early.
Notice:
1、 DNA distribution must be tested before you intend to use one of those parts;
2、 Meet up between teams and parts interchange needed to be paid attention to;
3、 Souvenirs are recommended;
4、 Preparation of team T-shirt and mascot;
5、 Pamphlets are really necessary , portable, convenient and practical .
Help Issues:
1、 the bacterial genes and genetic circuits implementation;
2、 some help about experimental skills and techniques;
3、 can provide temperature sensitive strains if needed.
Project: Microfluidic technology application of antibiotics detection in the milk
3rd May 2014 Meet Up with BIT-China
Reason for losing the high award:
1、 for lack of time;
2、 for lack of experience;
3、 for lack of awards.
Notice:
1、 Pay close attention to official website;
2、 the subject should be in line with international ideas and the international market;
3、 Show every work during the event, and sum up experience.
Art design:
1、 logo、poster、wiki、pamphlets should share the same style, keeping the fluency and clarity of thought;
2、 pamphlets :for the use of information interchange; also can be a nice way to deepen mutual understanding between the teams;
3、 small gifts should also be considered and designed.
Domestic propaganda:
1、introduction of the synthetic biology, iGEM competition, and previous teams;
2、communication between teams and substantial help should be done.
Help Issues:
1、 to help us process a kind of chips of which the channel is 50μm;
2、 help to complete moulding and chips processing;
3、 to do some related experiment in exchange lab.
Project: Introduction of the concept of Synthetic Biology and the meaning of iGEM
21st Sep. 2014 Briefing Session in university
To make iGEM and Synthetic Biology be known to more people, especially the students who are major in biology and who intend to engage in biology in the coming future, BUCT-China came to Beijing University of Chemical Technology to deliver a report to the lower grades.
Before the report, we also took some other measures like designing our poster and delivering leaflets. Besides, A wide range of activities in social networks as MicroBlog and WeChat are organized.
In the keynote, six of our team members introduced the contents and the meaning of synthetic biology in turn; explain the iGEM concept and previous iGEM competitions, along with the Giant Jamboree. Also, we made necessary introduction of our own project, which was really of great popularity among the junior.
After the report, there were still some students interested in the competition and asked several questions like the ideas about synthetic biology as far as they were concerned and the information of how to join in the next year’s iGEM competition. Obviously they got known much more. Meanwhile, we’d got a lot ideas from the audience. Without hesitation, we’ve made it a success as far as the meaning and the adequate influence. Our team members also gained more confidence of ourselves.
Click to view Beijing institute of technology invitation