Team:Cambridge-JIC/Guide/Constructs/Reporters

From 2014.igem.org

The following fluorescent proteins have been used with consistent results in MP: [Venus] [MRFP1] [eGFP].

A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: [N7] [LTI].

These can be added using a [Linker] instead of the stop codon for the fluorescent protein.

These are clearly visible under fluorescent microscopes and confocal microscopy. Sample images include:

<img: LTI>

<img: N7>

Fluorescent transformants are visible first from 3-5 days after transformation.

[GUS] staining has been successfully used to characterise expression, as seen in: