The Arizona State University iGEM team
Ethanol Production Parts
To optimize ethanol production in our EtOH strain, we wanted to overexspress the pyruvate decarboxylase (pdc) and alcohol dehydrogenase B(adhB) enzymes in order to maximize the amount of ethanol being produced. The iGEM registry contained the part BBa_k1122676, made by Edinburgh in 2013. This part was compared to another plasmid containing pdc/adhB, donated by Dr. David Neilsen. The results indiciated that the BBa_k1122676 was the better ethanol producer, making it the key component of the EtOH strain. Registry page: http://parts.igem.org/Part:BBa_K1122676
Fatty Acid Production Parts
In order to maximize the amount of fatty acetyl-coenzyme A molecules being produced in the fatty acid strain, two enzymes were targeted for overproduction. The first was the thioesterase A (TesA) protein, which takes the fatty Acyl-ACP produced from glycolysis and produces free fatty acid chains. The part BBa_K654058 codes for the TesA protein, but it did not have the required components for controllable expression. In order to do this, a promoter and an RBS were planned to be added upstream of this coding sequence. The RBS was successfully inserted, but we were unable to insert the promoter in time for the part submission.
The second part
Biodesiel Synthesis Parts
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