Team:SUSTC-Shenzhen/Notebook/A-B Toxin/Nickel Column Recovery

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Team SUSTC-Shenzhen

Notebook

Element of an endeavor


A-B Toxin

2014.10.6 to recover the nickel column recovery buffer: 0.2M HAc, 8M Urea, PH 4.0

  1. 2CV recovery buffer
  2. 5CV ddH2O
  3. 3CV 2% SDS
    3CV ddH2O
  4. 1CV 25%ethanol
  5. 1CV 50%ethanol
  6. 1CV 75%ethanol
  7. 1CV 100%ethanol
  8. 1CV 75%ethanol
  9. 1CV 50%ethanol
  10. 1CV 25%ethanol
  11. 3CV ddH2O
  12. 5CV 0.1M EDTA
  13. 5CV ddH2O
  14. 5CV 0.1M NiCl2
  15. 3CV ddH2O
  16. 2CV revovery buffer
  17. 2CV loading buffer

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Licensed under CC BY 4.0.

  • ©2014 SUSTC-Shenzhen

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