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Team:UCC Ireland/Team
From 2014.igem.org
Ian Valentine Mc Dermott
3rd Biochemistry
- Cloning
- Assisting with recombinant hagfish protein expression and isolation
- Construction of thread (α and γ subunits) Biobricks
Timothy O'Flynn
3rd Genetics
- Cloning plasmid
- Isolating subunits
- Bullying Ian
- Growing Bacterial Cultures
- Analysing the Data
Gavin King
3rd Biochemistry
- Identification of potential HPV16 target sequences
- Evaluating methods of producing HPV16 detector plasmid
- Phosphorylation of HPV16 target sequences and primers
- Annealing of phosphorylated HPV16 target sequence strands
- Assisted in cloning HPV16 detector into pSB1C3 plasmid
Dan Collins
3rd Genetics
- Identification of potential HPV16 target sequences
- Evaluating methods of producing HPV16 detector plasmid
- Assisted in cloning HPV16 detector into pSB1C3 plasmid
Russell Banta
3rd Material Chemistry
- Solubility studies on natural threads
- SEM, AFM, Microscopy (natural + synthetic)
- Isolation + polymerisation of subunits
- Development of threads from synthetic and natural proteins
- Initial development of upscaling production to feasible yields
Shama Chilakwad
3rd Genetics
- Preparing the plasmids for cloning
- Amplifying hagfish DNA
- Cloning and expression of the hagfish proteins
- Growing bacterial cultures
- Analysing all the data gathered for the SeaDNA project
Leanne O'Sullivan
2nd Biomedical Sciences
- Assisting in constructing SeeDNA detector prototypes
- Evaluating SeeDNA detecting ability with chosen targets
- Evaluating antimicrobial properties of natural hagfish slime
Patrick Xie
4th Engineer
- Website Creation
- Lab Assistant
- Conducting chemical experiments on natural protein
- Development of homemade spin coater
Cian O'Donnell
1st Biochemistry
- Assisting with hagfish protein expression and isolation
- Growing bacterial cultures
- Researching and conducting chemical experiments on natural protein
- Russell's psychologist
