Team:BYU Provo/Notebook/Auxotrophy/febapr

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BYU 2014 Notebook

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Week of March 9th

March 12, 2014

--CB TR-- Researched possible kill switch options for N. multiformis. Focused on the environment of the bio-reactor to control spread of N. multiformis.

Originally we considered designing a trigger that is specific to the environment of the bioreactor that, when removed from the environment would express a gene that would kill the bacteria. However we realized that such a design would be problematic due to the high mutation rate of N. multiformis.

Week of March 16th

March 17, 2014

--CB TR-- Researched articles on the environment of waste water treatment plants and the possible genes we could knock out to prepare for our presentation on N.multiformis metabolism optimization.

Focused our efforts on a unique gene knock out that would make N. multiformis reliant on the environment of the bio-reactor to grow.

March 18, 2014

--CB TR-- Reviewed all of our literature findings so far. In doing so, identified the specific focuses for our group: inserting the denitrification genes into N. multiformis, making N. multiformis more resistant to pH changes, and making N. multiformis more resistant to heavy metals.

March 19, 2014

--CB TR-- Presented our ideas for improving the metabolism of N. multiformis and received feedback from the class on them. Confirmed plan to insert the denitrification pathway into N. multiformis. Decided to forego other original goals and instead insert genes that would break down antibiotics.

March 20, 2014

March 21, 2014

Week of March 23rd, 2014

March 24, 2014

--CB TR-- Continued researching auxtrophy ideas.

March 26, 2014

March 28, 2014

Week of March 27th

March 31, 2014

April 1, 2014

April 2, 2014

April 3, 2014

April 4, 2014

Week of April 12th, 2014

April 7, 2014

April 9, 2014

April 11, 2014

Week of April 19th

April 14, 2014

Week of April 26th

April 21, 2014

Our team prepared a semester final on our N.multiformis Serine Auxotrophy Process.