Team:ZJU-China/Tools

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GS-Box



GS-Box

An useful bioinformatics tool for Gene-Socket





Every excellent technique should be simple and efficient to use! As an excellent technique, gene-socket also does well in this respect------ it has its' own optimization tool: GS-BOX. GS-BOX is a bioinformatics tool which is designed for customs to build their own gen-sockets easily and efficiently. What need the customs do with the tool? Just input the gene circuit sequences and then GS-BOX will design the experimental process automatically and every detail that customs will need to use!



  • Service

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  • Design Principle

    Homeodomains:
    1.Don't have similar sequences with E.coli (to avoid off-target effects)
    2.Don't have similar sequences with custom's parts (to avoid off-target effects)
    3.Fit the subsequent steps' requiring

    Primer:
    1.GC content & TM value
    2.Duplex formation and hairpin
    3.proper length

  • Design Method

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    Mauris sed mauris. Praesent imperdiet, nunc ut sollicitudin hendrerit, nisi tellus mollis leo, blandit pharetra nulla orci id eros. Nullam ut nunc. Praesent lacus lacus, tempor a, dignissim eu, tristique ac, tortor. Sed faucibus. Integer eleifend lacus ac neque. Fusce tempus. In hac habitasse platea dictumst. Nulla arcu neque, gravida at, rhoncus id, pharetra vitae, tortor. Aenean vestibulum consequat augue.

  • How to use

    If you just want to build a normal gene circuit, input your mount of parts and sequences; If your circuit contains some particular parts(for example, an terminator primer or want to make your parts seamless connection),please choose the "special mode" according to your situation.

  • Contact

    If you have any questions when using GS-Box, please contact us.





DIY YOUR OWN GENE-SOCKET




Step 1

(each no less than 20 bp)


Please provide each part's sequences:

Step 2

Please select the mode


If you don't know what these modes mean, please read "How to use" carfully. Are you sure you have selected the right mode?

You shuld learn that your mode is for all of your parts. If you just want to change only one of them, Please see the "Help". Are you sure this mode suits for most of your parts?

Step 3