"

From 2014.igem.org

Results / Heavy metals / Arabidopsis thaliana T4MBP

Labwork

Transformation of A. thaliana with our T4MBP:

1. Exchange the original promoter enTCUP2 of the binary vector pORE_E3 (AY562536.1) into a 2x35s promoter using this protocol.
2. We integrated our T4MBP in our modified pORE_E3_2x35S.
3. Application of our floral dip method.
4. After 6 weeks we harvested transgenic seeds from A. thaliana and plated these seeds on selection MSO-media.
5. At last we potted transformed plants.

Results

The exchange of the promoter was checked by sequencing. We achieved to regenerate young A. thaliana after the transformation with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.

Fig. 2: Pictures showing the vector pORE_E3 with 2x35S promoter and the way of exchanging the enTCUP2 promoter with the 2x35S promoter.