Team:Hannover/Protocols/Detection/Confocal

From 2014.igem.org

Revision as of 15:22, 15 October 2014 by E k o (Talk | contribs)

Protocols / How To: Confocal Microscopy

Material:
Prepared MS Samples
Confocal Microscope(here:Eclipse TE2000-E, Nikon, Japan)

Settings

  1. 100 x amplification
  2. For excitation of fluorescence for confocal pictures using lasers with λ= 408 nm and λ= 488 nm
  3. Transmission microscopy: halogen lamp
  4. Detection of GFP signals: mercury lamp






Retrieved from "http://2014.igem.org/Team:Hannover/Protocols/Detection/Confocal"