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INPNC-MVN-mRFP

Main Page-BBa_K1378005

BBa_K1378005 is a composite part coding INPNC-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.

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MlrA

Main Page-BBa_K1378001

MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in E.coli and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to Protein Phosphatase 1.

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Part List

Parts for Killing Improvement

BBa_K1378003 is the coding sequence of lectin Microvirin.

BBa_K1378005

BBa_K1378007

BBa_K1378009

Parts for Degradation

BBa_K1378001 is the coding sequence of protease MlrA which can specifically cleavage Microcystin(MC).

BBa_K1378022 is the coding sequence of MlrA fused with PelB secretion tag.

BBa_K1378023 is the coding sequence of MlrA fused with TorA secretion tag.

BBa_K1378024 is the coding sequence of MlrA with terminator.

BBa_K1378025 is the coding sequence of PelB-MlrA fusion protein with terminator.

BBa_K1378026 is the coding sequence of TorA-MlrA fusion protein with terminator.

Parts for Suicide

BBa_K1378031 is the coding sequence of cytoplasmic membrane protein Holin.

BBa_K1378032 is the coding sequence of peptidoglycan hydrolase Endolysin.