Team:Saarland/Test
From 2014.igem.org
The following speed cycle program was used:
Step | Temperature [°C] | Time [s] |
---|---|---|
1. Initial Denaturation | 98 | 30 |
2. Denaturation | 98 | 15 |
3. Annealing | 60 | 15 |
4. Extention | 75 | 20/kb |
5. Final Extention | 72 | 120 |
6. storage | 4 | for ever |
30 cycles
Impressum/Copyright