Team:ITB Indonesia/Safety
From 2014.igem.org
[http://www.example.com link title]
SAFETY
1. Your Training
- Have your team members received any safety training yet?
- Please briefly describe the topics that you learned about (or will learn about) in your safety training.
- Please give a link to the laboratory safety training requirements of your institution (college, university, community lab, etc). Or, if you cannot give a link, briefly describe the requirements.
Yes, we have already received safety training.
- Safety Equipment
- Waste Management
- Recombinant DNA Technology and Biological Safety
Laboratory Assistant, College students and Technician
2. Your Local Rules and Regulations
- Who is responsible for biological safety at your institution? (You might have an Institutional Biosafety Committee, an Office of Environmental Health and Safety, a single Biosafety Officer, or some other arrangement.) Have you discussed your project with them? Describe any concerns they raised, and any changes you made in your project based on your discussion.
- What are the biosafety guidelines of your institution? Please give a link to these guidelines, or briefly describe them if you cannot give a link.
- In your country, what are the regulations that govern biosafety in research laboratories? Please give a link to these regulations, or briefly describe them if you cannot give a link.
Our institution has a committee dealing with environmental security, health, safety, and (K3L). But they did not concerned in biosafety of modified organisms. In addition, our biosafety committe (K3L) deals only in laboratory safety and environment security.
http://www.che.itb.ac.id/safety
http://www.tf.itb.ac.id/files/2011/11/SOP-K3-Laboratorium-Teknik-Fisika.pdf
http://www.tf.itb.ac.id/files/2011/11/KEPUTUSAN-sop-kedaruratan.pdf
Yes, our country does. It can be accessed in http://indonesiabch.or.id/tentang-bkkhi/
3. The Organisms and Parts that You Use
- Species name (including strain)
- Risk Group
- Risk Group Source
- Disease risk to humans?
- Part number/name
- Natural function of part
- How did you acquire it?
- How will you use it?
Escherichia coli DH5alpha and BL21 (DE3)
Risk Group 1
NIH http://osp.od.nih.gov/sites/default/files/NIH_Guidelines.html#_Toc351276313
E. coli may irritate the skin, eyes, respiratory tract, blood circulation, and kidney. And also may cause nausea or vomiting nausea or vomiting.
BBa_K936024 ; BBa_K936000
BBa_K936024 = to convert ethylene glycol into glycolate ; BBa_K936000 = hydrolyses cutin
BBa_K936024 = requesting from HQ ; BBa_K936000 = from distribution kit
BBa_K936000 = to degrade PET into terephthalic acid and ethylene glycol ; BBa_K936024 = to convert the product of PET degradation