Team:Tuebingen/Notebook/Protocols/transformation

Transformation of E. coli

Procedure

1. Add desired volume of DNA (not more than 1/10 volume, for transformation of ligations: add complete 10 μL ligation reaction) to the 100 μl-culture in microreaction-tube.
2. Incubate 30 min on ice.
3. Heat shock for 45 s at 42°C.
4. Incubate 10 min on ice.
5. Add 0.9 ml of culture medium (no AB added) and let the bacteria grow at 37°C for 2h.
6. Plate on LB plates + antibiotic and incubate at 37°C o/n.