Team:Tuebingen/Notebook/Protocols/3A assembly
From 2014.igem.org
Three part assembly
Digestion
Reagents
242 g | Tris |
57.1 mL | pure acetic acid |
0.5 M | EDTA 0.5 M pH 8.0 |
Procedure
Mix all the reagents and add to 1000 ml with distillated water. Adjust to pH 8.3. Store at room temperature.