Team:Tuebingen/Notebook/Protocols/miniprep

From 2014.igem.org

Revision as of 23:10, 14 October 2014 by SvenB (Talk | contribs)


Preparation of plasmids on a small scale

Procedure

  1. Pick a single colony from a plate, give it in a a 15 ml Falcon-tube with 10 ml LB-medium and incubate at 37 °C and overnight.
  2. Pelletize 3 ml of the overnight-culture and discard the supernatant.
  3. Continue according to the Quiagen QIAprep ® Spin Miniprep Kit Manual or the Biozyme EasyPrep ® Pro Plasmid Miniprep Kit Manual.
  4. Store at – 20 °C.

 

Kits

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/miniprep"