Team:NTU Taida/Circuit2

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NTU-Taida

Skin Whitening ---PKEK

  • Inspiration :

    Uneven skin pigmentation is a significant cosmetic concern, and the identification of topically applicable molecules to address this issue is of general interest. One of the most often used skin lighteners is hydroquinone or the hydroquinone glycoside of arbutin. However, these compounds have a cytotoxic effect on melanocytes and have an irritating effect on the skin. There is thus an increasing need for improved active ingredients for the treatment of hyperpigmentation, but also for the purely cosmetic lightening of relatively large areas of pigmented skin that are entirely appropriate to the individual skin.

    The tetrapeptide PKEK (Pro-Lys-Glu-Lys) is described in the patent application WO/2008/085494 and in WO/2009/068351. It has immunomodulatory properties and also anti-aging efficacy.

    According to researches, PKEK may be delivered upon topical application into human skin where they subsequently exert biological effects on skin cells, including epidermal keratinocytes and dermal fibroblasts effects.

  • Synthetic Peptides Sequence :

    Here are three kinds of signal sequence:
    1.PelB (66mer) ATG AAA TAT CTG CTG CCG ACC GCG GCG GCG GGC CTG CTG CTG CTG GCG GCG CAG CCG GCG ATG GCG

    2.OmpA (63mer) ATG AAA AAA ACC GCG ATT GCG ATT GCG GTG GCG CTG GCG GGC TTT GCG ACC GTG GCG CAG GCG

    3.PhoA (63mer) ATG AAA CAG AGC ACC ATT GCG CTG GCG CTG CTG CCG CTG CTG TTT ACC CCG GTG ACC AAA GCG

    Unlike others, we have our own way to synthesize the signal sequence.

    It’s common to order whole gene sequences to Biotech, but the cost is always shocking, and since the budget limitation, we strive to find our way out.

    Here is our strategy:

    First, we ordered 4 primers which can combine to each other, and put them into the plasmid backbone.

    Second, we link the iGEM’s official prefix and suffix (EcoRI, XbaI, SpeI and PstI) to our signal sequence, and choose XbaI、SpeI as restriction sites.

    Illustrations can help you understand:

    1.PelB (66mer)

    GAATTC GCGGCCGC T TCTAG ATG AAA TAT CTG CTG CCG ACC GCG GCG GCG GGC CTG CTG CTG CTG GCG GCG CAG CCG GCG ATG GCG T ACTAGT A GCGGCCG CTGCAG

    CTTAAG CGCCGGCG A AGATC TAC TTT ATA GAC GAC GGC TGG CGC CGC CGC CGC GAC GAC GAC GAC CGC CGC GTC GGC CGC TAC CGC A TGATCA T CGCCGGC GACGTC

    2.OmpA (63mer)

    GAATTC GCGGCCGC T TCTAG ATG AAA AAA ACC GCG ATT GCG ATT GCG GTG GCG CTG GCG GGC TTT GCG ACC GTG GCG CAG GCG T ACTAGT A GCGGCCG CTGCAG

    CTTAAG CGCCGGCG A AGATC TAC TTT TTT TGG CGC TAA CGC TAA CGC CAC CGC GAC CGC CCG AAA CGC TGG CAC CGC GTC CGC A TGATCA T CGCCGGC GACGTC

    3.PhoA (63mer) GAATTC GCGGCCGC T TCTAG ATG AAA CAG AGC ACC ATT GCG CTG GCG CTG CTG CCG CTG CTG TTT ACC CCG GTG ACC AAA GCG T ACTAGT A GCGGCCG CTGCAG

    CTTAAG CGCCGGCG A AGATC TAC TTT GTC TCG TGG TAA CGC GAC CGC GAC GAC GGC GAC GAC AAA TGG GGC CAC TGG TTT CGC A TGATCA T CGCCGGC GACGTC

  • Testing Result :

  • Background Knowledge :

    Regulation of skin pigmentation is a highly comp process that in addition to melanin-synthesizing melanocytes involves the production of pigmentation-inducing soluble factors by epidermal keratinocytes including proinflammatory cytokines such as IL-6, IL-8 as well as alpha-melanocyte-stimulating hormone (a-MSH)

  • Reference :

    • Retrieved from "http://2014.igem.org/Team:NTU_Taida/Circuit2"