How to synthesize anti-sense constructs

Anti-sense fragments were synthesized based on BioBrick by PCR. Forward primers are common. Each reverse primers are different. Because of these, we got various length anti-senses. The sides of antisense fragment have restriction enzymes XhoI, NcoI sites. We finished synthesizing anti-sense RNA, we ligated each anti-senses with H-stem vector by restriction enzyme XhoI, NcoI.

Therefore, we got as90, as120. As the same way, we made as30, as60 in H-Stem System and Anti-sense B0034 examination. We performed repression examination by using their 4 anti-sense constructs.