Team:HokkaidoU Japan/Notebook/H Stem/Replacement To 1A3

From 2014.igem.org

Revision as of 09:18, 14 October 2014 by Osamu (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

H-Stem Vector Exchange to pSB1A3

  • Start

  • mini-Prep

    1A3 Vector

  • PCR

    Plac-stem-as-stem-dT on 1C3 with 100up-F & 200dn-R

  • Digestion

    1A3 with EcoRI & PstI, Plac-stem-as-stem-dT with EcoRI & PstI

  • Ligation

    1A3 & Plac-stem-as-stem-dT

  • Transformation

    Plac-stem-as-stem-dT on 1A3

  • Complete!

Retrieved from "http://2014.igem.org/Team:HokkaidoU_Japan/Notebook/H_Stem/Replacement_To_1A3"