Overview

Antisense RNA is studied actively over the world. However, reliable method for gene silencing has not been clear. It is hard to find efficient sequence of antisense RNA and to synthesize new antisense fragments every time you change target gene.

fig1. you have to change antisense RNA by each target gene.

We had a good idea! it is useful to use common antisense for different target gene.

fig2. Image of common antisense RNA effects.

Here we found that antisense-RBS(B0034) fragment, that is used by igemer commonly, work to silence several proteins. We synthesized antisense B0034 fragment, igemer often use that RBS. Regardless of target gene, only one antisense fragment works various proteins on common RBS, B0034.

We also synthesized antisense-B0032 fragment in order to achieve specific silencing gene. The combination of antisense fragment and RBS, that locates upstream of target gene makes change silencing protein.

Specific antisense-RBS fragment help you save labor to make new antisense RNA for each target genes. Fortunately, igem HokkaidoU team select tractable RBS for designing antisense-RBS fragments. You can use our antisense fragments without resynthesize constructs you had. You only have to do is add our antisense fragment to construct had target gene!!

fig3. Antisense B0034 has specific effects to B0034, RBS.