Team:Minnesota/Templates

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UMN iGEM 2014

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MNtallica: Cleaning Up Heavy Metal!

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Project



Mercury is a neurotoxic heavy metal with the ability to biomagnify, therefore it is a significant issue in public health and environmental studies worldwide. Its levels are continually on the rise due to copper, nickel, and gold mining activities, the industrial use of mercury catalysts, mercurial fungicides in agriculture, and the burning of fossil fuels. This has resulted in the pollution of many marine ecosystems and water reservoirs worldwide, the cleanup of which using current technology, is either not feasible or incredibly costly. This study describes the use of engineered recombinant bacteria to facilitate the biological remediation of the neurotoxin methylmercury and hazardous mercury ions from an aquatic target site into less toxic form. This synthetic microbe was incorporated in novel encapsulation technology within a cost-effective, scalable water filtering column. The employment of this device could rigorously change the practices used in mercury decontamination efforts as well as pave the way for the switch to biological rather than chemical processes. Furthermore, this technology can be applied towards bioremediation and biosensing of various other heavy metals and organic toxins in the environment.

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Dry Lab

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Wet Lab

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Meet The Team






Policies & Practices

Safety in the Lab

Attributions


Wet Lab: Mercury Project Design: Basem, Aunica Mercury Ion Testing: Sarah, Cassandra, Camilo, Srijay, Jennifer, Suzie, Aunica Methylmercury Testing: Nater Lab, Nicholas, Srijay, Patrick, Suzie, Basem, Aunica Cadmium, Zinc, Copper project design: Basem, Stephen, Aunica Cadmium, Zinc, Copper Testing: Cassandra, Jessica, Aunica Kill Switch Biosafety Proposal: David, Sarah, Camilo, Basem pDU1358 received from Dr. Anne O. Summers, University of Georgia pSB74 received through addgene from Keasling Lab Composite parts: mer operon: Primer design: Basem, Stephen Parts cloning: Basem, Jennifer, Valeriu phsABC: Primer design: Basem, Stephen Parts cloning: Basem, Valeriu Single parts: merR: Primer design: Basem, Stephen Parts cloning: Cassandra merT: Primer design: Basem, Stephen Parts cloning: Sarah, Jennifer merP: Primer design: Basem, Stephen Parts cloning: Camilo, Logan merA: Primer design: Basem, Stephen Parts cloning: Valeriu, Jessica Characterization: Cassandra, Sarah merB: Primer design: Basem, Stephen Parts cloning: Logan, David Chassis Transformations: Pseudomonas putida: Basem Shewanella oneidensis: Basem E. coli K12: Basem, David EncapsuLab: Encapsulation Protocol Design: Srijay, Patrick, David, Nicholas Cell encapsulation: Nicholas, Patrick, Srijay, David, Basem, Suzie Cell Viability Testing: Patrick, David, Nicholas SEM encapsulation imaging: Nicholas, UofM imaging center Device design: Roxana, Nicholas Mathematical modelling: Di, Zhiyi, Patrick, David Policies and Practices: Outreach, presentations, public perception studies School Curriculum design: Basem, Suzie Science Museum Curriculum Design: Everybody Middle School Classroom outreach: Jess, Basem, Cassandra, Jennifer, Suzie Science Museum outreach: Jess, Jen, David, Sarah, Cassandra, Basem, Srijay, Di, Holly, Logan 3M presentation: Suzie, Basem, Cassandra, Stephen, Jess Cargill presentation: ??? State Fair outreach: tabling & survey: Cassandra Taylor Jess Jen Basem Suzie Nicholas Stephen Roxana Di Srijay Patrick Holly Logan Survey statistics: Taylor slideshow: Jess, David, Logan giveaways: CBS, local businesses gift cards, Rob Rakow survey content: Everyone State Fair game show: Cassandra, Taylor Multicultural Student Association Collaboration: Jessica Colombia collaboration: (magnetic stirrer) Stephen Ethics : Blog: Basem, Cassandra, Logan, Jen Documentary: Interview Questions, Content: Jennifer, David, Colombia iGEM team Camera, Editing, Production: Connor Gleason Business Plan: Justin, Tanner, Basem, Tamara Economic Analysis: Justin, Tanner Intellectual Property Rights and Patenting Basem, Office of Technology Commercialization Wiki development Design: Mari, Chris, Aaron, Tanner, Basem Icons: Mari Figures development: Mari, Basem, Nicholas Coding, CSS, javascript: Aaron, Chris, Content: Everybody Lab notebook: Sarah Lucas Poster: Basem Team Logo Nicholas Administrative forms, IP, safety: Basem Parts Submission form & shipping Stephen Public relations and team contact Basem, Jessica Grant writing, fundraising Basem, Jess, David, Cassandra

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