Team:NCTU Formosa/Notebook/test7

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2014/10/8 Sequencing 1. PBAN NcoI 2. Pcons+RBS+SalI -1 3. Pcons+RBS+SalI-11 4. Pcons+RBS+NheI-6 5. Pcons+RBS+xhol-2+RBS+BFP+TER 6. Pcons+RBS+SacI-11+RBS+BFP+TER 7. Pcons+RBS+ Nhel-20+RBS+BFP+TER

Ligation Member:Mick Pcons+RBS+NcoI on psb3c5 Pcons+RBS+NcoI on psb3c5

2014/10/9

Cultivation at 12:00

ALL Nhel-9(10/7)x2 ALL SacI-11(10/7)x1 ALL xhol-2(10/7)x1

Electrophoresis The first piece of gel

1. maker 2. Pcons+RBS+NheI-20+RBS+BFP+TER 3. Pcons+RBS+Hind-1+RBS+BFP+TER 4. Pcons+RBS+KpnI-1+RBS+BFP+TER 5. Pcons+RBS+Ndel-1+RBS+BFP+TER 6. Pcons+RBS+NcoI-5+RBS+BFP+TER 7. Pcons+RBS+AseI ALL 8. PBAN NcoI-12 9. Pcons+RBS+NheI-6 10. Pcons+RBS+Hind-1 11. Pcons+RBS+NcoI-20 12. Pcons+RBS+NcoI-2-2 13. Pcons+RBS+Ndel-2-1 14. Pcons+RBS+Nedl-2-2 15. Pcons+RBS+SacI-1 16. Pcons+RBS+SacI-11 17. Maker

The second piece of gel Member: Chris Ho

1. maker 2. Pcons+RBS+NheI-11 3. Pcons+RBS+Hind3 4. Pcons+RBS+KpnI-7 5. Pcons+RBS+SacI-16 6. Pcons+RBS+SalI-12 7. Pcons+RBS+Xhol

Start at 2:15 1 .Pcons+RBS+SacI-3 to psb3c5 2. Pcons+RBS+KpnI-1 to psb3c5 3. Pcons+RBS+NcoI-1 to psb3c5

Mini-prep and sequencing 1. ALL NheI-2 x1 2. ALL SacI-11 x1 3. ALL Xhol-2 X1

2014/10/10 Transform Start at 8:45 Pcons+RBS+Ndel on psb3c5 Pcons+RBS+NcoI on psb3c5

Electrophoresis 1. maker 2. Pcons+RBS+SacI-3 on psb3c5 3. Pcons+RBS+KpnI-1 on psb3c5 4. Pcons+RBS+NcoI-1 on psb3c5

2014/10/10 Transform Start at 8:45 Pcons+RBS+Ndel on psb3c5 Pcons+RBS+NcoI on psb3c5 Electrophoresis 1. maker 2. Pcons+RBS+SacI-3 on psb3c5 3. Pcons+RBS+KpnI-1 on psb3c5 4. Pcons+RBS+NcoI-1 on psb3c5

2014/10/12 PCR and Electrophoresis 1. Pcons+RBS+NcoI+psb3c5 2. Pcons+RBS+Ndel+psb3c5

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