Safety Training in our team

Kill Switch in E.coli

This year our safety work is based on E.coli, since some of our work is on light sensing and imaging system in E.coli. To make a wider application in the future, the biosafety of E.coli containing these parts should be concerned.

LALF is

Anti-LPS factor(LALF) regulated by lacI

We designed the part based on K541545. We found that R0010 might keep express genes below without regulation.Reason may be that Lacl expressed by E.coil is limited. So we inserted parts that express Lacl and constitutive promoter before the old one to inhibite pLac.

Anti-LPS factor(LALF) regulated by IPTG

This part is a cell-kill system. Firstly,constitutive promoter could express enough Lacl that is useful for downstream. Secondly, the reporter in the middle of our part wouldn't show signal nor the cell be killed by the part behind unless IPTG or lactose added into the culture medium. No IPTG or lactose: GFP don't express ,G- won't be killed. IPTG or lactose exsisting : GFPs express ,G- will be dead.

G-: Gram negative bacteria