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Team:NJAU China/Modeling
From 2014.igem.org
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Modeling
We use the software to describe the whole idea of the experiment.Experiment was divided into three parts, and finally combined into two paths. The first part consists of copA promoter ,GFP, GshF and T7 promoter,enhancing Escherichia.coli sensitivity to copper ion. Glutathione can be synthesized by GshF, which can unifies both necessary reactions in one platform. The second part is a sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. Finally, an automatically and efficiently copper-preventing guard are hoped to built. An overview of the biochemical process is shown in Figure 1. The figure is drawn with CellDesigner2 4.4.
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