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Lab Protocols Characterization Protocols

Lab Protocols PCR Agarose Gel Electrophoresis and DNA Gel Purification PCR Product cloning Competent E. coli Cells using Calcium Chloride Transformation in E. coli DH5-alpha Plasmid preparation by alkaline lysis and SDS Cohesive-end assembly cloning Restriction Analysis and Digestion in large scale Competent Bacillus subtilis cells Transformation of Bacillus subtilis DSM medium for sporulation Luria-Bertani (LB) medium Cell counting with Neubauer Chamber

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