Team:Hannover/Results/GFP
From 2014.igem.org
Results / GFP Photos
To avoid cytotoxic effects on the plant caused by the heavy metals we decided to place T4MBP to the cell exterior. With a secretion signal (Expa4) the T4MBP is directed to the extracellular space. The protein is tethered to the cell surface by using a cellulose binding domain (CBD). Via the cellulose binding domain the protein is attached to the cell wall.
Labwork
- CBD fused with mGFP using our EMP protocol
- we used pEarleyGate with the sequenz of mGFP5 as a template to generate our megaprimer
- we used our GenArt Vector (with the sequenz of a 5'UTR and the EXPA4 signal peptide followed by the T4MBP, a tag and the CBD) as the target plasmid
- .....