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Team:INSA-Lyon/formation
From 2014.igem.org
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PROJECT
PROJECT- PROJECT SUMMARY
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WETLAB
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MODELING
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HUMAN PRACTICE
HUMAN
PRACTICE- HUMAN PRACTICE SUMMARY
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TEAM
CURLI FORMATION
As functional amyloid fibers biosynthesis is still not totally understood, there are not many models other than descriptive sketches that represent the curli formation. From these observations we decided to gather the information we could and build models from them as incomplete as they may be, in order to provide future teams working on engineered CsgA with a basis to start from.
We therefore were able to build up two models:
- the CurLy'On Simulator, a computed simulation of CsgA secretion and polymerisation that, provided with the right parameters, could make for a good alternative to a mathematical model for a protein kinetics study;
- the implementation of the only two mathematical models we could find in the litterature that seemed relevant (with biological justification) in describing in vitro CsgA polymerisation in the C language in a fashion that can be given to a numerical solver, as these models require a heavy calculation power.
