Team:Brasil-SP/Project/ResponseModule

     We have chosen the GFP as a reporter gene to responding our kidney sensing because the facility to found in our laboratory, where all our professors use it in the lab for their researches. We could use another one, like YFP (yellow fluorescence protein) or RFP (red florescence protein) for exemple.

     A blood sample from a healthy person, which has low levels of circulating Cystatin C, when in contact with B. subtilis cells triggers the expression of the reporter gene GFP in the biodetector, resulting in high intensity of fluorescence signal. However, when a blood sample from a ill person, wich has high levels of circulating Cystatin C, contacts B. subtilis cells induce the minimum of fluorescence signal, or doesn’t induce the production of GFP. In this way, the intensity of the fluorescence signal show us the diagnostic of the person, if he is sick or healthy, based in a negative signal.