Team:HIT-Harbin/Design

In order to make yeast be capable of detecting dioxins in the environment, we designed the circuit above. TEF is the constitutive promoter which can activate downstream sequences to express dual domain protein, namely, lexA-DBD/mDR83-805. mDR83-805 can express dioxin receptor hsp90. And after it combining intracellular dissociative dioxin, it will pass through karyotheca and combine with cyc1 promoter to activate the expression of green fluorescent protein downstream. Then we can know by observation whether there is dioxin in the environment or not.

Considering the amount of dioxin in the environment is in micro level or even trace level, when the concentration of dioxin decreases, those who can successfully combine with hsp90 and then nucleic DNA will be less. Thus, we added a feedback control to amplify the signal intensity.