Team:EPF Lausanne/Parts

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PARTS

Parts submitted by the 2014 EPFL iGEM team

Our team submitted a total of 54 Biobricks (biobricks 51 and 52 do not exist).

Biobrick What it is Function Why do we use it? Group
BBa_K1486000 CpxR coding sequence Transcription factor To make most of our biobricks! Bacteria
BBa_K1486001 CpxR under arabinose promoter Treanscription factor Bacteria
BBa_K1486002 PAra + sfGFP CpxR [Nterm] Expresses fused protein Test CpxR expression & Ara promoter Bacteria
BBa_K1486003 Flexible linker Attaches two proteins together Bacteria
BBa_K1486004 Flexible linker Attaches two proteins together Bacteria
BBa_K1486005 PAra + CpxR sfGFP [Cterm] Expresses fused protein Test CpxR expression & Ara promoter Bacteria
BBa_K1486006 IFP[1] N terminus of split IFP Bacteria
BBa_K1486007 IFP[2] C terminus of split IFP Bacteria
BBa_K1486008 CxpR & Split IFP1.4 [Cterm + Cterm] Two CpxR CDS, each C terminus attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486009 CxpR & Split IFP1.4 [Nterm + Nterm] Two CpxR CDS, each N terminus attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486010 CxpR & Split IFP1.4 [Nterm + Cterm] Two CpxR CDS, each attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486011 CxpR & Split IFP1.4 [Cterm + Nterm] Two CpxR CDS, each attached to a moiety of IFP Characterize CpxR dimerization Bacteria
BBa_K1486012 CpxR + IFP[1] CpxR with the Nterm moiety of IFP attached at its C terminus Intermediate & control Bacteria
BBa_K1486013 CpxR + IFP[2] CpxR with the Cterm moiety of IFP attached at its C terminus Intermediate & control Bacteria
BBa_K1486014 IFP[1] + CpxR CpxR with the Nterm moiety of IFP attached at its N terminus Intermediate & control Bacteria
BBa_K1486015 IFP[2] + CpxR CpxR with the Cterm moiety of IFP attached at its N terminus Intermediate & control Bacteria
BBa_K1486016 fLuc[1] N terminus moiety of the firefly luciferase Bacteria
BBa_K1486017 fLuc[2] C terminus moiety of the firefly luciferase Bacteria
BBa_K1486018 PAra + fLuc[1] + fLuc[2] Split firefly luciferase under arabinose promoter Control Bacteria
BBa_K1486019 rLuc[1] C terminus moiety of the renilla luciferase Bacteria
BBa_K1486020 rLuc[2] N terminus moiety of the renilla luciferase Bacteria
BBa_K1486021 PAra + rLuc[1] + rLuc[2] Split renilla luciferase under arabinose promoter Control Bacteria
BBa_K1486022 rLuc Full renilla luciferase Control Bacteria
BBa_K1486023 Yeast sfGFP Superfolder GFP for yeast cells Reporter Yeast
BBa_K1486024 Kan Yeast kanamycin resistance gene Selection marker Yeast
BBa_K1486025 ADH1 terminator Terminator Yeast
BBa_K1486026 Yeast sfGFP + Kan Yeast sfGFP attached to the yeast kanamycin resistance gene Control the expression of pbs2 Yeast
BBa_K1486027 rLuc + Kan Renilla luciferase attached to the kanamycin resistance gene Yeast
BBa_K1486028 Yeast sfGFP[1] N terminal moiety of split yeast sfGFP Yeast
BBa_K1486029 sfGFP[1] + kan Nterm moiety of split yeast sfGFP attached to yeast kanamycin resistance gene Yeast
BBa_K1486030 rLuc[1] + kan Nterm moiety of split renilla luciferase attached to yeast kanamycin resistance gene Yeast
BBa_K1486031 Ura CDS for Uracil (yeast selective purposes) Confer resistance to Uracil-deprived medium Yeast
BBa_K1486032 Yeast sfGFP + Ura Yeast sfGFP attached to the Uracil CDS Control the expression of hog1 Yeast
BBa_K1486033 rLuc + Ura Renilla luciferase attached to the Uracil CDS Control the expression of hog1 Yeast
BBa_K1486034 yeast sfGFP[2] C terminal moiety of split the yeast sfGFP Yeast
BBa_K1486035 yeast sfGFP[2] + Ura Cterm moiety of split yeast sfGFP attached to the Uracil CDS Yeast
BBa_K1486036 rLuc[2] + Ura Cterm moiety of split renilla luciferase attached to the Uracil CDS Yeast
BBa_K1486037 linker Attaches two proteins together Yeast
BBa_K1486038 sfGFP[1] N terminus moiety of split superfolder GFP Bacteria
BBa_K1486039 sfGFP[2] C terminus moiety of split superfolder GFP Bacteria
BBa_K1486040 sfGFP[1] + CpxR N terminus moiety of split sfGFP attached to CpxR Bacteria
BBa_K1486041 sfGFP[2] + CpxR C terminus moiety of split sfGFP attached to CpxR Bacteria
BBa_K1486042 LZip Monomer of leucine zipper TF Bacteria
BBa_K1486043 LZip + split rLuc Two Leucine Zipper monomers, each attached to a different split rLuc moiety Control for split rLuc assays Bacteria
BBa_K1486044 mut IFP[1] Biobrick-compatible IFP[1] Bacteria
BBa_K1486045 mut IFP[2] Biobrick-compatible IFP[2] Bacteria
BBa_K1486046 CpxR promoter FW CpxR binding-region in forward direction Bacteria
BBa_K1486047 CpxR promoter RV CpxR binding-region in reverse direction Bacteria
BBa_K1486048 CpxR reporter Calgary's CpxR reporter repaired (sequence was missing) To see when CpxR is active Bacteria
BBa_K1486049 CpxR promoter FW + RFP Reporter of CpxR Test the direction of the complete CpxR promoter Bacteria
BBa_K1486050 CpxR promoter RV + RFP Reporter of CpxR Test the direction of the complete CpxR promoter Bacteria
BBa_K1486053 Linker 10 amino-acid linker Attach CheY/Z to split luciferases Bacteria
BBa_K1486054 CheY/CheZ rLuc CheY and CheZ, each attached to a moiety of split renilla luciferase Positive control for the split rLuc Bacteria
BBa_K1486055 CheY/CheZ fLuc CheY and CheZ, each attached to a moiety of split firefly luciferase Positive control for the split fLuc Bacteria
BBa_K1486056 CxpR & Split mut IFP1.4 [Cterm + Cterm] Two CpxR CDS, each C terminus attached to a moiety of the biobrick-compatible IFP Characterize CpxR dimerization Bacteria


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