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Wetlab

A core concept of iGEM is the collaboration in an interdisciplinary student team. Accordingly, students of different age, gender and field of study came together to found our team in Aachen, in order to realize our project with joint forces and enthusiasm for the overall goal. Working closely together, we therefore not only have the chance to learn from each other, but also to excel ourselves. Using our collective creativity and common responsibility, we will create something special in order to make our contribution to synthetic biology.

April

1st

Organization

where to get:

key cards and keys for the lab
cooled centifuge
space in -80 °C
bottles for 70 % ethanol
... (problems of a first year team ^^)

May

1st

gel with M - full -full - REACh1 SOE3.2 - REACH2 SOE3.2 - M
→ 120 V, 30 min
→ cut out the bands

June

3rd

transformation of 34 BioBricks

4th

preparation of consumables:
- fresh 50 % glycerol
- new LB plates with cam and with kanamycin (kan)
- 60 glas tubes
- 2 L LB^-
- 100 mL steril glas beads for plating

master plates (6 clones per BioBrick) were made

July

1st

transformation efficiency kit is broken

→ run an agarose gel with the kit plasmids to check for nuclease activity/ bands

inventory of the -80°C freezer was done

2nd

overnight cultures (LB) of K731520 and K1319042 for chips were inoculated

August

1st

made electrocompetent E.coli rosetta cells.
prepared cultures of K1319042 and K131026

2nd

tested the OD measurement device and compared it to the spectrophotometer and the plate reader.
tested K131026 and K1319042 for fluorescence in the plate reader
did a heat shock transformation of I746909 into NEB TOP 10 cells
did an electroshock transformation of pET17-Gal3 into E.coli rosetta

September

1st

5 ml cultures of K1319003 and K1319004
plasmid prep

Plasmid	DNA [ng/µl]
J23101.K516032 pSB1K3	23.5
J23115.K516032 pSB1K3	20.5
J04450 pSB1A3	57.5
J04450 pSB1K1	63.5

over night cultures of K131026 in DH5α and NEB

October

1st

Preparations for sensor-chip production on the following day (2014-10-02) was done accoringly to the sensor chip manufacturing protocol:
- At 18:30 we prepared over-night cultures from K1319042, B0015 and K131026 by inoculating 250 ml Erlenmeyer flasks each containing 50 ml LB medium . The flasks were incubated for ~12 hours at 37 °C on a shaker.

Team:Aachen/Notebook/Wetlab2