Team:British Columbia/Modelling/Mutagenesis
From 2014.igem.org
Mutagenesis
The flow diagram shows the basic work flow for directed evolution of a gene. After plating the cells, each cell will grow into a colony. During cell growth, the error prone polymerase is not turned off so the colony grown up for sequencing will also contain mutations which could interfere with sequencing.
The aim of this model is to determine the error rate at which a standard size colony would become too diverse for the sequence of the initial cell to be determined.
A probabilistic matlab model was created to simulate the mutation process that occurs as a single cell grows into a colony. The model begins with the root of a binary tree which represents the single cell being grown into a colony. The basics of the model is illustrated below.