OD/F device

Measuring Optical Density (OD) is a central element in microbiological work and synthetic biology in general. Often the qeustion is, how many cells are in a suspension. The OD can give you a hint.

Commercial OD meters cost several hundred dollars ([http://www.laboratory-equipment.com/laboratory-equipment/cell-density-meter.php OD meter]), and can limit the spread of synthetic biology. Especially for BioHack-Spaces, DIY laboratories and schools we wanted to develop an alternative.

With our OD/F device we want to enable many to people for good, precise and cheap science.

Especially for the Interlab Study also fluorescence has been of importance. Here the correlation between OD and fluorescence should be measured. Since the taks of measuring OD and fluorescence are often performed at the same time, we want to present a device that can measure both with easy changes. Finally we can tell you, how much fluorescence there is per cells.

OD device

F device

Getting your Device

Table 1: Number of pieces, components and costs for building your own OD/F device

number of pieces	components	costs [$]	order e.g. from
1	arduino UNO R3	12.66
2	light to frequency sensor TSL 235R	5.90
1	display 2x16 l2C	18.97
2	LEDs	2.50
1	taster	5.23
1	filter slide	5.20
20	jumper-wire-cable	2.36
2	small breadboards	3.00
1	power supply	5.00
1	case	20.24
2	cuvettes-holder	15.95
-	odds and ends like header sockt/pins	2.52	-
-	total	99.53	-

Building your own OD/F device

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	First take the cut casing. We really recommend using a laser cutter.
	Attach the cuvette-holder holders such that the cuvette holder is placed directly under the opening hole.
	Next build the lid of the device. At this stage you can already mount the button. We recommend to glue any parts.
	Your lid finally should look like this.
	Next we want to assemble the cuvette holders. On the side with the square hole attach the light-to-frequency sensor with glue. For the OD case place the orange LED opposite, or for fluorescence, the LED in the hole in the bottom. Make sure to close any remaining open hole!
	Your final assembly should then look like this.
	As the case can be used for both, fluorescence and OD measurement, we use a combined plug. Just three header rows (7 pins) and connect them as we did.
	Now we're doing the wiring. Connect the Arduino 5V and GND such that you have one 5V and one GND line on your breadboard.
	Then connect the button to 5V on the one side, and to GND via a resistor on the other side. Connect this side also to port __ on your Arduino. This will sense the blank. Next connect the display to the Arduino and our connector. See the Fritzing diagram at the bottom for a detailed information.
	Now put everything into the case and ...
	... also place the cuvette holder into the device. Attach the display to the device lid and close the casing.
	Congratulations! You have finished constructing your own OD/F device!

Team:Aachen/OD/F device

From 2014.igem.org

OD/F device

OD device

F device

Getting your Device

Building your own OD/F device