Penn State iGEM 2014 Notebook Page
Here you will find weekly summaries of our wet laboratory progress, team updates, and accomplishments outside the laboratory. Below [link this] is our detailed, day-to-day progress laboratory notebook.
Weekly Summaries
will create links to each week, but will just list down the page Week 1, Week 2, etc etc
Laboratory Notebook
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Biodetoxification |
Codon Optimization |
| Wednesday, May 21, 2014 |
Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA |
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| Thursday, May 22, 2014 |
Ashlee and Emily performed a transformation |
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| Friday, May 23, 2014 |
We picked several colonies for overnight growth to do more cloning tomorrow |
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| Saturday, May 24, 2014 |
Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion |
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| Monday, May 25, 2014 |
Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination. Ordered |
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| Tuesday, May 26, 2014 |
Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate |
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| Wednesday, May 27, 2014 |
Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow |
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| Thursday, May 28, 2014 |
Prepared plasmid containing the HMF pathway; inoculated LB broth with Lambda Red Recombinase plasmid from cryogenic storage |
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| Friday, May 29, 2014 |
Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid |
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