Team:NCTU Formosa/Notebook/test6

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7/1 Ligation of [J23101+B0032+PSB1C3] Cr (G1) Digestion of [B0032] Ar Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/2 Transformation of ligation product [B0032+K592100+J61048] Ar and [J23101+B0032] Cr (G1) Cultivation of [B0032+BFP+J61048] Cr in LB broth (G2)


7/3 Mini-prep of [B0032+BFP+J61048] and then digestion: (XP) Electrophoresis of plasmid and digestion products of[B0032+BFP+J61048]Cr (XP) (G2)

7/4 Digestion of [T7 promoter](SP) (G2) Cultivation of [J23101] Ar (G3)

7/5 PCR:[B0032+K592100+J61048*11] Ar (G1) Electrophoresis of PCR products (G1) Ligation of [J23101+B0032+PSB1C3*3] Cr (G1) Electrophoresis of plasmid and digestion products:[T7promoter](SP),[B0031+BFP+J61048](XP) Ligation:[T7 promoter])(SP)+[B0032+BFP+J61048](XP) (G2)

7/6 Transform the ligation products made at 7/5 (G2)

7/7 Transformation of [J23101+B0032*5] Cr (G1) Cultivation of [B0032+K592100+J61048*2] Ar (G1) Cultivation of [J23101] Ar (G3) Digestion of [B0015] Ar (XP), [J61048] Ar (XP) (G3)

7/8 Mini-prep of [B0032+K592100+J61048*2] Ar and then digest only one of two: (XP) (G1) PCR:[T7+B0032+BFP+J61048] (G2) Digestion of [J23101] Ar (ES) (G3)

7/9 Cultivation of [B0032+BFP+J61048]Cr and [T7+B0032+BFP+J61048]Kr(G2)



5/19 Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)

5/20 Transform of [T7polymerase] Kr and [PSB1K3] Kr (G2) Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2) Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1) Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)

5/21 Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2) Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2) Electrophoresis of digested product and plasmid [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1) Ligation of [K592100+J61048+PSB1K3] Kr (G1)

5/22 Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)

5/23 Transform of ligation product [K592100+J61048+PSB1K3]Kr (G1)

5/24 Digestion of [J61048] Ar (XP) (G2) PCR: [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2) Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)


5/27 Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2) Cultivation of [PSB1K3*3] Kr(G2)

5/28 PCR:[PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1) Mini-prep of [PSB1K3*3] Kr(G2) Digestion of [PSB1K3*3] Kr (EP) (G2) Electrophoresis of plasmid and digested [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)

5/29 Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) Transform of [J23101] Ar (G1) Transform of [T7 promoter] Ar Kr (G2) Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2) Ligation of [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2) Ligation of [Plac+B0032+PSB1K3] Kr (G3)

5/30 Transform of ligation product (G2) Transform of ligation product [Plac+B0032+PSB1K3] Kr (G3)


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