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Team:Kyoto
From 2014.igem.org
Project Overview
Generating cloud. This is our project aim. You might think we are dreaming, but, we found a clue in some scientists’ words. In wetlands or sea, specific marine bacteria and coral produce dimethyl sulfide (DMS), which becomes a Cloud Condensation Nuclei (CNN), we thought we could produce cloud by creating E.coli which synthesizes DMS. To realize this, we consist a biosynthetic pathway of DMS in E.coli by introducing genes from Fragilariopsis cylindrus and Ruegeria pomeroyi. We use DMS detecting tube and High Performance Liquid Chromatography (HPLC) to confirm the function. Don’t you think it is fantastic if the small organism, E. coli, creates something big, cloud? And this project may have the possibility to solve environmental problems.
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Project Overview
We're dreaming of keeping E. coil in hand; moving them here and there. Magneto.coli will realize it. A magnetosome is an organelle seen in magnetotactic bacteria; storing magnetite in it. We believe E. coil with a magnetosome can be moved by magnetism . To make magetosomes in E. coil, at least two processes are required; vesicle formation and uptake of iron in it. It had been unclear what genes were necessary for them in E. coil. We focused on vesicle formation through this project, and found mamL/Q, derived from Magnetospirillum magnetotacticum, played an important role in vesicle formation. We did detect organelles in mamL/Q-induced E. coil.
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