Team:Penn State/Notebook

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Penn State iGEM 2014 Notebook Page

we should include weekly summaries at the head of each week (need to break up this table structure but for now it is fine) - but I think day by day lab stuff is also good (2012 igem did this)

Biodetoxification
Codon Optimization
Wednesday, May 21, 2014
Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA ...................
Thursday, May 22, 2014
Ashlee and Emily performed a transformation
Friday, May 23, 2014
We picked several colonies for overnight growth to do more cloning tomorrow
Saturday, May 24, 2014
Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion
Monday, May 25, 2014
Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination. Ordered
Tuesday, May 26, 2014
Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate
Wednesday, May 27, 2014
Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow
Thursday, May 28, 2014
Friday, May 29, 2014
Ashlee and Emily made cryogenic storage of the Lamda Red Recombinase plasmid