Penn State iGEM 2014 Notebook Page
we should include weekly summaries at the head of each week (need to break up this table structure but for now it is fine) - but I think day by day lab stuff is also good (2012 igem did this)
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Biodetoxification |
Codon Optimization |
| Wednesday, May 21, 2014 |
Emily's first experience with cloning! Ashlee led Emily through several practice experiments from designs made earlier in the year: making a gel, loading samples, gel purifying DNA |
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| Thursday, May 22, 2014 |
Ashlee and Emily performed a transformation |
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| Friday, May 23, 2014 |
We picked several colonies for overnight growth to do more cloning tomorrow |
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| Saturday, May 24, 2014 |
Memorial Day Weekend? How about lab cloning weekend! Ashlee conducted plasmid preparation and digestion |
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| Monday, May 25, 2014 |
Due to a string of failed clonings with the broadhost vector pSEVA251 and the large inserts, new designs are evaluated! Instead of creating a plasmid with the HMF pathway (7.5 kb) and dCas9 system (5.5 kb), we shall add the HMF pathway and dCas9 to the P. putida genome using homologous recombination. Ordered |
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| Tuesday, May 26, 2014 |
Inoculated LB broth with ampicillin and dCas9 plasmid from cryogenic storage; inoculated Lb broth with chloramphenicol and FTV vector from Ashlee's past experiment; streaked the HMF vector on a kanamycin plate |
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| Wednesday, May 27, 2014 |
Emily and Ashlee made cryogenic storage of the dCas9 plasmid; plasmid prepared the FTV and dCas9 vectors; digested FTV vector; inoculated LB broth with a colony from the HMF plate for overnight growth and plasmid preparation tomorrow |
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