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Human Practices

Presentation to NEWBio Teachers - Penn State Center for Science and the Schools

Engineering a Metabolic Pathway

In this simulation, colored beads were poured through each set of clear tubes. The beads represented chemicals or molecules entering a pathway which would alter them. The tubes represented the pathways which consisted of different genes being expressed at different levels. The first scenario represents a properly engineered pathway where the output of both genes is similar. This model allowed the beads to flow through the tubes with a constant pace. Our second scenario represented what can happen when a gene in the pathway is over-expressed. Here, the first gene was expressing at almost double what the second gene could take in. This caused the beads to build-up in the first tube while only allowing a small amount through the rest of the pathway.

Assembling a Plasmid Activity

Presentation to Science-U High School Students