Team:Bielefeld-CeBiTec/Results/CO2-fixation
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CO2 fixation
The second module aims to change E.coli in a way that it binds carbon dioxide like an autotroph organism. For this we want to establish the Calvin-cycle. E.coli has all enzymes for the Calvin-cycle except of three.
The different results for all three enzymes are mentioned in the Calvin-cycle section. One important step for the carbon dioxide fixation is the RuBisCO (Ribulose 1,5-bisphosphate carboxylase/oxygenase). For E.coli we decided to transform genes for the carboxysome because of its properties.
Here you will find information about the execution of our experiments.