$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}}
\newcommand{\Stabi}{\small Stabi}$
$\newcommand{\EColi}{\small E.coli}
\newcommand{\SCere}{\small S.cerevisae}\\[0cm]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\PI}{\small PI}$
$\newcommand{\Igo}{\Large\mathcal{I}}
\newcommand{\Tgo}{\Large\mathcal{T}}
\newcommand{\Ogo}{\Large\mathcal{O}}
~$
Example of a hierarchical menu in CSS
Host: E.coli
Source: ccdBA operon
Plasmid: available using LabGenius BioBrick Mapper
Length: 309 bp
Sequence: (begin) atgcagttt ... atataataa (end)
Compatibility: RFC[10], RFC[12], RFC[21], RFC[23], RFC[25].
Characterization
In order to characterize the ccdB biobrick, we sent the biobricks to sequencing and made a $\small screen$ $\small of$ $\small activity$ for the protein ccdB. We did a $\small killing$ $\small assay$, because of the toxic property of ccdB.
We constructed $\small 4$ $\small different$ $\small colonies$ including one with the plasmid pKK-233-ccda, another with pBAD33-ccdB, a third with both, and a control colony. The ccdA gene encoded for a protein wich acts as an anti-toxin of ccdB.
On the first media containing $\small IPTG$ (inducing the pKK233’s expression) and $\small glucose$ (repressing the pBAD's expression), each colony grew. That allowed us to control the non toxicity of ccdA.
On the media containing both IPTG$ and arabinose (inducing the pBAD's expression), the strand with pBAD was killed and the strand with both ccdA $\small\&$ ccdB grew.
We made dilution to assure that the cell concentration didn’t affect the toxicity or the anti-toxicity.
"
- Université Libre de Bruxelles -
