You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
Week 1 6/22~6/28
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α) Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt) Practice presentation Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
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