"

From 2014.igem.org

{

 "timeline": {
   "headline": "Lab Milestones",
   "type": "default",

"text": "

The steps to success.

",

   "asset": {
     "media": "",
     "credit": "iGEM SUSTC-Shenzhen",
     "caption": "Lab Milestones"
   },
   "date": [
     {
       "startDate": "2014,7,27",
       "endDate": "2014,7,28",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Fundamental/TransEfficiency.htm'>Effiency Test</a>",

"text": "

DH5α&BL21

",

       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "ProfFan",
         "caption": "Figure 1"
       }
     },
     {
       "startDate": "2014,8,17",
       "endDate": "2014,8,22",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-puromycin-for-cell-selection'>Determining the appropriate concentration of puromycin for cell selection</a>",

"text": "

Prepare for selection

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,7,18",
       "endDate": "2014,7,18",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-II-for-plasmid-pX330-EGFP'>Plasmid amplification II for pX330 & EGFP & verifications by electrophoresis</a>",

"text": "

Prepare for selection

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Electrophoresis Snapshot"
       }
     },
     {
       "startDate": "2014,8,9",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Enzyme-digestion-agrose-gel-DNA-Extraction-for-PX330-vector'>Enzyme digestion & agrose gel DNA Extraction for PX330 vector</a>",

"text": "

To prepare PX330 vector for the following ligation experiments

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,6",
       "endDate": "2014,8,6",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/5*UAS-plasmids-Fill-in'>5*UAS plasmids Fill-in</a>",

"text": "

To eliminate the EcoRI site on the 5*UAS plasmid

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,10",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/PCR-for-mCherry'>PCR for mCherry</a>",

"text": "

To get our target fragment\nof mCherry by PCR

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,9",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-second-time'>UAS Fill-in (The second\ntime)</a>",

"text": "

The same as the first Fill-in experiment

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,11",
       "endDate": "2014,8,11",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Struggles-for-our-second-time-Fill-in'>Struggles for our second-time Fill-in</a>\n",

"text": "

A real challenge, indeed.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,11",
       "endDate": "2014,8,11",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-plasmid-PX330-MCherry-GAL4-PX330-MCherry-amplified-2014-8-9-by-restriction-digestion'>Verification for plasmid PX330-MCherry-GAL4,PX330-MCherry (amplified on 2014.8.9) by restriction digestion</a>",

"text": "

Failure, as it is.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,12",
       "endDate": "2014,8,12",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-verification-of-plasmid-PX330-MCherry-GAL4'>Follow-up for verification of plasmid PX330-MCherry-GAL4 and The results of 'Struggles for fill-in(the second time)'</a>",

"text": "

Life full of cycles, but each iteration with happiness.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,12",
       "endDate": "2014,8,12",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-Struggles-for-fill-in-the-second-time'>Follow-up for 'struggles for our second-time Fill-in'</a>",

"text": "

Failure, again.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,13",
       "endDate": "2014,8,13",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-The-third-time'>UAS Fill-in (The-third-time)</a>",

"text": "

Then try for another time.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,7,17",
       "endDate": "2014,7,19",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-I-for-plasmid-pX330-EGFP'>Plasmid amplification I for plasmid pX330 & EGFP</a>",

"text": "

Prepare the necessary materials

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     }
   ],
   "era": [
     {
       "startDate": "2014,7,27",
       "endDate": "2014,8.7",
       "headline": "Basic Research",

"text": "

The Fundamentals

",

       "tag": "Fundamental"
     }
   ]
 }

}